OneFlow

Gentle, 3D culture spheroids, barcoding,...

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Introduction to the OneFlowTM

The OneFlowTM is the entry level of our product lineup,
featuring the gentle encapsulation module, the high-end imaging and the full power of LiveDrop’s intuitive software.
No compromise on what matters.
It can be upgraded at any point to a ModaFlowTM, widening the possibilities.

Basically, OneFlowTM is a
compact encapsulation system
designed for high-throughput and
highly standardised
droplets production.

  1. High-throughput (co-)encapsulation up to 3 elements ( e.g.: single or multiple cell type, culture medium, reagents, magnetic beads, ... ).
  2. Droplets from 15 µm Ø, (~2 pL) to over 65 µm Ø, (~150 pL).
  3. No dead volume.
  4. Co-encapsulation of miscellaneous elements
    (single or multiple cell type, culture medium, reagents,
    magnetic beads, barcodes, etc.).
  5. Live image and video control.
  6. Up to 50 M droplet/hour ( 14k droplet/ second)

Ideal for...

  • Spheroids standardised manufacturing : > 20.000 spheroids in less than 3 minutes.
  • Rare or fragile cell sample preparation.
  • Cell co-encapsulation with reagents, such as barcoding, etc.

OneFlow Short Application Notes

OneFlow technical specifications

General description
  1. Upgradable system with sorting or analytical optics (up to 5 colours).
  2. Ready for Sterile Work (closed capot, easy-to-sterilise surfaces, etc.).
  3. Small footprint (fits in a standard laminar flow hood).
  4. User-friendly programming interface for experiment settings, automation, or data transfer.
  5. Touch screen + web app accessible through your network with laptops, tablets, phones.
  6. HD Camera for live QC picture or video capture.
  7. Motorised xyz stage for fine positioning and automation.

and more

  • Integrates an inverted microscope. The magnification is tuneable for visualisation with different objectives: 4x and 10x in the standard version (motorised), with an option for 20x (for single-cell, microbes, etc.).
  • Real-time visualisation of ongoing experiments, along with recording and snapshot features. It can capture up to 2,000 frames per second.
  • Flows are driven with pressurised reservoirs, with four independent pressure inlets up to 2 bars (eight inlets optionally available).
  • The sample stage features a chip holder adapted to LiveDrop's cartridge and other standard glass slide formats (26 x 76mm).
  • The touchscreen interface allows users to connect the chip to the reservoirs, inject the sample, and run experiments seamlessly, with just two hours of learning.
  • Small footprint of 0,3 sqm.
  • More specifications are available upon request.